Peptide Lab
Peptide LabResearch
SpecimenSPC-GIGA-26

GIGAVLKVLTTGLPALISWIKRKRQQ

Streaming
Sequence Map26 residues · IUPAC
1G
Glycine
pos 1 · Gly · special
hydropathy -0.4
→ coil
I
Isoleucine
pos 2 · Ile · nonpolar
hydropathy 4.5
→ coil
G
Glycine
pos 3 · Gly · special
hydropathy -0.4
→ β-strand
A
Alanine
pos 4 · Ala · nonpolar
hydropathy 1.8
→ β-strand
V
Valine
pos 5 · Val · nonpolar
hydropathy 4.2
→ α-helix
L
Leucine
pos 6 · Leu · nonpolar
hydropathy 3.8
→ β-strand
K
Lysine
pos 7 · Lys · basic
hydropathy -3.9
→ β-strand
V
Valine
pos 8 · Val · nonpolar
hydropathy 4.2
→ β-strand
L
Leucine
pos 9 · Leu · nonpolar
hydropathy 3.8
→ β-strand
10T
Threonine
pos 10 · Thr · polar
hydropathy -0.7
→ β-strand
T
Threonine
pos 11 · Thr · polar
hydropathy -0.7
→ β-strand
G
Glycine
pos 12 · Gly · special
hydropathy -0.4
→ coil
L
Leucine
pos 13 · Leu · nonpolar
hydropathy 3.8
→ coil
P
Proline
pos 14 · Pro · special
hydropathy -1.6
→ α-helix
A
Alanine
pos 15 · Ala · nonpolar
hydropathy 1.8
→ β-strand
L
Leucine
pos 16 · Leu · nonpolar
hydropathy 3.8
→ α-helix
I
Isoleucine
pos 17 · Ile · nonpolar
hydropathy 4.5
→ β-strand
S
Serine
pos 18 · Ser · polar
hydropathy -0.8
→ β-strand
W
Tryptophan
pos 19 · Trp · aromatic
hydropathy -0.9
→ β-strand
20I
Isoleucine
pos 20 · Ile · nonpolar
hydropathy 4.5
→ β-strand
K
Lysine
pos 21 · Lys · basic
hydropathy -3.9
→ α-helix
R
Arginine
pos 22 · Arg · basic
hydropathy -4.5
→ α-helix
K
Lysine
pos 23 · Lys · basic
hydropathy -3.9
→ α-helix
R
Arginine
pos 24 · Arg · basic
hydropathy -4.5
→ α-helix
Q
Glutamine
pos 25 · Gln · polar
hydropathy -3.5
→ α-helix
Q
Glutamine
pos 26 · Gln · polar
hydropathy -3.5
→ α-helix
α-helixβ-strandcoil
Chou–Fasman · window 5 · per-residue confidence
Physicochemical Signatureformula C131H228N38O32
26 residues
Molecular weight
2847.49Da
monoisotopic 2845.7381 Da
Isoelectric point
12.51
q(7.4) +4.99e
GRAVY
0.273
Net hydrophobic
Hydrophobic moment
1.229
Amphipathic α-helix
Instability index
34.7
Stable in vitro
Aliphatic index
135.0
Thermostability proxy
ε at 280 nm
5,500M⁻¹·cm⁻¹
with S-S 5,500
Boman index
0.57
Low PPI affinity
AI Investigation
claude-opus-4-7 · temp 0.4 · streaming
LIVE
connect→ anthropic.messages.streamok
dispatch→ 26-residue probeok
model→ claude-opus-4-7ok
awaiting first token
HydropathyKyte–Doolittle · window 7
range -3.98 … 2.25
hydrophobic ↑position →hydrophilic ↓
Net charge vs pHHenderson–Hasselbalch · pI 12.51
-0.9 … 6.0 e
cationic ↑isoelectric point pI = 12.51anionic ↓
Secondary Topology
Chou–Fasman ensemble
α-helix
9 / 2635%
β-strand
13 / 2650%
coil
4 / 2615%

Per-residue prediction with confidence encoded via opacity. Window size 5; assignments are relative — not absolute — and should be cross-validated with PSI-PRED for publication.

Composition
12 unique
  • LeuL4 · 15.4%
  • GlyG3 · 11.5%
  • IleI3 · 11.5%
  • LysK3 · 11.5%
  • AlaA2 · 7.7%
  • ValV2 · 7.7%
  • ThrT2 · 7.7%
  • ArgR2 · 7.7%
  • GlnQ2 · 7.7%
  • ProP1 · 3.8%
  • SerS1 · 3.8%
  • TrpW1 · 3.8%
Helical Projection
100°/res · first 18
G1I2G3A4V5L6K7V8L9T10T11G12L13P14A15L16I17S18

Schiffer–Edmundson wheel · amphipathic if one face is uniformly colored

Homolog Discovery
7 hits · UniProtKB (mock)
AccessionDescriptionOrganismIdent.Sim.Cov.E-valScore
Z33594Pancreatic polypeptide-fold neuropeptideEscherichia coli K-1298.7%99.8%95%1e-95266
R92319Cationic host-defense peptideEscherichia coli K-1292.3%99.3%93%2e-93230
V66421Cell-penetrating arginine-rich segmentXenopus laevis84.8%91.8%92%5e-75211
A10684Glucagon-family hormone fragmentRattus norvegicus78.4%84.2%88%3e-70181
S30885Mitochondrial uncoupling fragmentPlasmodium falciparum74.5%84.1%87%5e-54167
P38503Cell-penetrating arginine-rich segmentStaphylococcus aureus67.1%71.6%84%8e-47147
J70975Tachykinin neuropeptide precursorPlasmodium falciparum63.5%69.2%76%9e-41139
Structural Candidates
PDB · MMseqs2
  • PDB 5AZC
    chain B · X-ray · 3.14 Å
    TM-score
    0.94
  • PDB 5EBG
    chain D · X-ray · 2.81 Å
    TM-score
    0.83
  • PDB 3SIF
    chain B · X-ray · 3.47 Å
    TM-score
    0.73
Predicted topology
α-helix
35%
β-strand
50%
coil
15%
Literature Cross-Referencein-silico citation graph
5 synthetic refs · not for clinical use

  • Pharmacokinetics of a 2847 Da peptide candidate in murine models

    Singh, P., Schneider, H.

    J. Biol. Chem.·vol 193·pp 2848-2864·2018

    PMID 40014854
    DOI 10.7340/j.2018.61286
    relevance 0.98

  • Structure-activity relationship of a 2847 Da cationic peptide on bacterial membranes

    Hassan, N., Tanaka, R., Lee, A., Patel, B., Lee, H.

    Peptide Science·vol 77·pp 2595-2612·2018

    PMID 32677579
    DOI 10.7443/peptide.2018.18381
    relevance 0.93

  • Membrane interaction landscape of arginine-rich cell-penetrating peptides

    Johansson, D., Rossi, V., Kobayashi, L.

    Biochim. Biophys. Acta - Biomembranes·vol 11·pp 5919-5927·2018

    PMID 36875904
    DOI 10.7430/biochim.2018.51966
    relevance 0.88

  • Comparative analysis of pI distributions in mammalian neuropeptidomes

    Hassan, D., Kim, N., Tanaka, E.

    J. Med. Chem.·vol 181·pp 9077-9090·2023

    PMID 37974494
    DOI 10.4295/j.2023.84114
    relevance 0.82

  • Membrane interaction landscape of arginine-rich cell-penetrating peptides

    Schneider, V., Nakamura, K., Nakamura, R., Lee, B.

    Biochim. Biophys. Acta - Biomembranes·vol 28·pp 4745-4756·2024

    PMID 35143483
    DOI 10.3351/biochim.2024.92251
    relevance 0.77
References synthesized for demonstration · cross-validate against PubMed before citing
healthyregion eu-west-1rtt 14 msworkers 4/4 idlequeue 0
build 2026.05.14.a7f3kernel 6.8.0-hep